ISO 15213-1:2023
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 1: Enumeration of sulfite-reducing Clostridium spp. by colony-count technique
This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
Foreword Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 4.1 General 4.2 Preparation of dilutions 4.3 Enumeration 4.4 Confirmation 5 Culture media and reagents 6 Equipment and consumables 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 General 9.2 Test portion, initial suspension and dilutions 9.3 Heat treatment to select spores 9.4 Inoculation and incubation 9.5 Enumeration of typical colonies 9.6 Confirmation of sulfite-reducing Clostridium spp. 10 Expression of results 11 Validation of the method 11.1 Interlaboratory study 11.2 Performance characteristics 12 Test report 13 Quality assurance Annex A (normative) Flow diagram of the procedure Annex B (normative) Culture media and reagents Annex C (informative) Performance characteristics of the method Annex D (informative) Special protocol for the enumeration of sulfite-reducing Clostridium spp. in feed Bibliography Food microbiology - Examination for specific organisms - Escherichia coli in bivalve molluscs - Rapid method Food microbiology - Examination for specific organisms - Listeria monocytogenes in dairy products Food microbiology - Examination for specific organisms - Food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Detection method Food microbiology - Examination for specific organisms - Vibrio parahaemolyticus
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Preparation of dilutions
4.3 Enumeration
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
9.3 Heat treatment to select spores
9.4 Inoculation and incubation
9.5 Enumeration of typical colonies
9.6 Confirmation of sulfite-reducing Clostridium spp.
10 Expression of results
11 Validation of the method
11.1 Interlaboratory study
11.2 Performance characteristics
12 Test report
13 Quality assurance
Annex A (normative) Flow diagram of the procedure
Annex B (normative) Culture media and reagents
Annex C (informative) Performance characteristics of the method
Annex D (informative) Special protocol for the enumeration of sulfite-reducing Clostridium spp. in feed
Bibliography