AS/NZS 1766.2.16.1:1998
Food microbiology - Examination for specific organisms - Food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Detection method
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AS/NZS 1766.2.16.1:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-FOOD AND ANIMAL FEEDING STUFFS-HORIZONTAL ....
PREFACE
CONTENTS
1 Scope
2 Normative references
3 Definitions
3.1 Listeria monocytogenes:
3.2 detection of Listeria monocytogenes:
4 Principle
4.1 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half Fraser broth
4.2 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.3 Plating out and identification
4.4 Confirmation
5 Culture media and reagents
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
6.2 Drying cabinet or incubator,
6.3 Incubators,
6.4 Water bath,
6.5 Loops,
6.6 pH-meter,
6.7 Test tubes or flasks,
6.8 Measuring cylinders,
6.9 Total-delivery graduated pipettes,
6.10 Petri dishes,
6.11 Jars,
6.12 Gas mixture
6.13 Equipment for the Henry illumination test (optional).
6.14 Microscope,
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.4 Plating out and identification
9.5 Confirmation of Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Catalase reaction
9.5.3 Gram staining
9.5.4 Motility test (if necessary)
9.6 Confirmation of L. monocytogenes
9.6.1 Haemolysis test
9.6.2 Carbohydrate utilization
9.6.3 CAMP test
9.7 Interpretation of morphological and physiological properties and of the biochemical reactions
9.8 Definitive confirmation
9.9 Control cultures
10 Expression of results
11 Test report
Annex A - Diagram of procedure
Annex B - Composition and preparation of culture media and reagents
B.1 Selective primary enrichment medium: Halt Fraser broth
B.2 Selective secondary enrichment medium: Fraser broth
B.3 First selective plating-out medium: Oxford agar
B.4 Second selective plating-out medium: PALCAM agar
B.5 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
B.6 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
B.7 Sheep blood agar
B.8 Carbohydrate utilization broth (rhamnose and xylose)
B.9 Motility agar
B.10 CAMP (Christie, Atkins, Munch-Petersen) medium and test strains
B.11 Hydrogen peroxide solution
B.12 Phosphate-buffered saline (PBS)
Annex C - HENRY ILLUMINATION TEST
ANNEX ZZ - ADDITIONAL REQUIREMENTS TO ISO 11290-1:1996 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
ZZ1 SCOPE
ZZ2 ADDITIONAL REQUIREMENTS
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AS/NZS 1766.2.16.1:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-FOOD AND ANIMAL FEEDING STUFFS-HORIZONTAL ....
PREFACE
CONTENTS
1 Scope
2 Normative references
3 Definitions
3.1 Listeria monocytogenes:
3.2 detection of Listeria monocytogenes:
4 Principle
4.1 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half Fraser broth
4.2 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.3 Plating out and identification
4.4 Confirmation
5 Culture media and reagents
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
6.2 Drying cabinet or incubator,
6.3 Incubators,
6.4 Water bath,
6.5 Loops,
6.6 pH-meter,
6.7 Test tubes or flasks,
6.8 Measuring cylinders,
6.9 Total-delivery graduated pipettes,
6.10 Petri dishes,
6.11 Jars,
6.12 Gas mixture
6.13 Equipment for the Henry illumination test (optional).
6.14 Microscope,
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.4 Plating out and identification
9.5 Confirmation of Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Catalase reaction
9.5.3 Gram staining
9.5.4 Motility test (if necessary)
9.6 Confirmation of L. monocytogenes
9.6.1 Haemolysis test
9.6.2 Carbohydrate utilization
9.6.3 CAMP test
9.7 Interpretation of morphological and physiological properties and of the biochemical reactions
9.8 Definitive confirmation
9.9 Control cultures
10 Expression of results
11 Test report
Annex A - Diagram of procedure
Annex B - Composition and preparation of culture media and reagents
B.1 Selective primary enrichment medium: Halt Fraser broth
B.2 Selective secondary enrichment medium: Fraser broth
B.3 First selective plating-out medium: Oxford agar
B.4 Second selective plating-out medium: PALCAM agar
B.5 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
B.6 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
B.7 Sheep blood agar
B.8 Carbohydrate utilization broth (rhamnose and xylose)
B.9 Motility agar
B.10 CAMP (Christie, Atkins, Munch-Petersen) medium and test strains
B.11 Hydrogen peroxide solution
B.12 Phosphate-buffered saline (PBS)
Annex C - HENRY ILLUMINATION TEST
ANNEX ZZ - ADDITIONAL REQUIREMENTS TO ISO 11290-1:1996 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
ZZ1 SCOPE
ZZ2 ADDITIONAL REQUIREMENTS
AS/NZS 1766.2.16.1:1998
Online Library subscription information
Online library subscriptions provide a cost-effective solution for organisations of any size to access the latest standards, offering 24/7 access to a personalised catalogue. The service ensures subscribers have have the most up-to-date version of selected standards, incluing automatic updates for any amendments or versions. Pricing is dependent on the chosen standards and the number of concurrent users, with discounts applied from the standards PDF price. For customised subscriptions or further inquiries, contact the Online Library team.