ISO 10272-1:2017
Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method
ISO 10272-1:2017 specifies a horizontal method for the detection by enrichment or direct plating of Campylobacter spp. It is applicable to
- products intended for human consumption,
- products intended for animal feeding,
- environmental samples in the area of food and feed production, handling, and
- samples from the primary production stage such as animal faeces, dust, and swabs.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.2.1 Detection procedure A
4.2.2 Detection procedure B
4.2.3 Detection procedure C
4.3 Isolation on selective solid medium
4.3.1 Detection procedure A
4.3.2 Detection procedure B
4.3.3 Detection procedure C
4.3.4 Detection procedure A, B and C
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion and initial suspension
9.2.1 General
9.2.2 Detection procedure A
9.2.3 Detection procedure B
9.2.4 Detection procedure C
9.3 Enrichment
9.3.1 Detection procedure A
9.3.2 Detection procedure B
9.4 Isolation
9.4.1 Detection procedure A
9.4.2 Detection procedure B
9.4.3 Detection procedures A, B and C
9.5 Confirmation of Campylobacter
9.5.1 General
9.5.2 Selection of colonies for confirmation
9.5.3 Examination of morphology and motility
9.5.4 Study of aerobic growth at 25 °C
9.5.5 Detection of oxidase activity
9.5.6 Interpretation
9.6 Identification of Campylobacter species (optional)
9.6.1 General
9.6.2 Detection of catalase activity
9.6.3 Detection of hippurate hydrolysis
9.6.4 Detection of indoxyl acetate hydrolysis
9.6.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report
Annex A (normative) Diagram of procedures
Annex B (normative) Culture media and reagents
Annex C (informative) Method validation studies and performance characteristics
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.2.1 Detection procedure A
4.2.2 Detection procedure B
4.2.3 Detection procedure C
4.3 Isolation on selective solid medium
4.3.1 Detection procedure A
4.3.2 Detection procedure B
4.3.3 Detection procedure C
4.3.4 Detection procedure A, B and C
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion and initial suspension
9.2.1 General
9.2.2 Detection procedure A
9.2.3 Detection procedure B
9.2.4 Detection procedure C
9.3 Enrichment
9.3.1 Detection procedure A
9.3.2 Detection procedure B
9.4 Isolation
9.4.1 Detection procedure A
9.4.2 Detection procedure B
9.4.3 Detection procedures A, B and C
9.5 Confirmation of Campylobacter
9.5.1 General
9.5.2 Selection of colonies for confirmation
9.5.3 Examination of morphology and motility
9.5.4 Study of aerobic growth at 25 °C
9.5.5 Detection of oxidase activity
9.5.6 Interpretation
9.6 Identification of Campylobacter species (optional)
9.6.1 General
9.6.2 Detection of catalase activity
9.6.3 Detection of hippurate hydrolysis
9.6.4 Detection of indoxyl acetate hydrolysis
9.6.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report
Annex A (normative) Diagram of procedures
Annex B (normative) Culture media and reagents
Annex C (informative) Method validation studies and performance characteristics
Bibliography