ISO 14501:2021
Milk and milk powder — Determination of aflatoxin M1 content — Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography
This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 μg/kg for milk powder and 0,005 μg/kg for liquid milk. The limit of quantification (LOQ) is 0,1 μg/kg for milk powder and 0,01 μg/kg for liquid milk.
The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.
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Forewords
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 General
8.2 Preparation of test samples
8.2.1 Milk
8.2.2 Milk powder
8.3 Immunoaffinity column preparation
8.4 Test sample purification
8.5 High performance liquid chromatography (HPLC)
8.5.1 Pump setting
8.5.2 Chromatographic performance
8.5.3 Calibration curve of aflatoxin M1
8.5.4 Analysis of the purified extracts and injection scheme
9 Calculation and expression of results
9.1 Skimmed milk
9.1.1 Calculation
9.1.2 Expression of results
9.2 Skimmed milk powder
9.2.1 Calculation
9.2.2 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Results of an interlaboratory trial
Bibliography
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Forewords
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 General
8.2 Preparation of test samples
8.2.1 Milk
8.2.2 Milk powder
8.3 Immunoaffinity column preparation
8.4 Test sample purification
8.5 High performance liquid chromatography (HPLC)
8.5.1 Pump setting
8.5.2 Chromatographic performance
8.5.3 Calibration curve of aflatoxin M1
8.5.4 Analysis of the purified extracts and injection scheme
9 Calculation and expression of results
9.1 Skimmed milk
9.1.1 Calculation
9.1.2 Expression of results
9.2 Skimmed milk powder
9.2.1 Calculation
9.2.2 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Results of an interlaboratory trial
Bibliography