ISO 13493:2021
Meat and meat products — Determination of chloramphenicol content — Reference method
This document specifies the liquid chromatographic (LC) method for the determination of chloramphenicol content of muscle tissue of meat, including livestock and poultry.
This document specifies the liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of chloramphenicol content of muscle tissue, casing, liver of meat and meat products, including livestock and poultry.
This document specifies LC-MS/MS as the reference method.
The LC method is suitable for the determination of chloramphenicol content greater than 6,5 mg/kg.
LC-MS/MS is suitable for the determination of chloramphenicol content greater than 0,1 μg/kg.
Test samples which have deteriorated cannot be analysed with this method.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Liquid chromatographic method
4.2 Liquid chromatography tandem mass spectrometry method
5 Sampling
6 Preparation of test sample
7 Test method of liquid chromatography
7.1 Reagents
7.2 Apparatus
7.3 Procedure
7.3.1 General
7.3.2 Test portion
7.3.3 Preparation of extract
7.3.4 Solid-phase extraction
7.3.5 Liquid-liquid extraction
7.3.6 Chromatographic analysis
7.4 Calculation
7.5 Precision
7.5.1 Interlaboratory test
7.5.2 Repeatability
7.5.3 Reproducibility
8 Test method of liquid chromatography tandem mass spectrometry method (reference method)
8.1 Reagents and materials
8.2 Apparatus
8.3 Procedure
8.3.1 General
8.3.2 Test portion
8.3.3 Preparation of extract
8.3.4 Solid-phase extraction
8.3.5 LC-MS/MS analysis
8.4 Calculation
8.5 Precision
8.5.1 Interlaboratory test
8.5.2 Repeatability
8.5.3 Reproducibility
9 Test report
Annex A (informative) HPLC chromatogram of chloramphenicol standard solution and LC-MS/MS chromatogram of chloramphenicol standard solution
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Liquid chromatographic method
4.2 Liquid chromatography tandem mass spectrometry method
5 Sampling
6 Preparation of test sample
7 Test method of liquid chromatography
7.1 Reagents
7.2 Apparatus
7.3 Procedure
7.3.1 General
7.3.2 Test portion
7.3.3 Preparation of extract
7.3.4 Solid-phase extraction
7.3.5 Liquid-liquid extraction
7.3.6 Chromatographic analysis
7.4 Calculation
7.5 Precision
7.5.1 Interlaboratory test
7.5.2 Repeatability
7.5.3 Reproducibility
8 Test method of liquid chromatography tandem mass spectrometry method (reference method)
8.1 Reagents and materials
8.2 Apparatus
8.3 Procedure
8.3.1 General
8.3.2 Test portion
8.3.3 Preparation of extract
8.3.4 Solid-phase extraction
8.3.5 LC-MS/MS analysis
8.4 Calculation
8.5 Precision
8.5.1 Interlaboratory test
8.5.2 Repeatability
8.5.3 Reproducibility
9 Test report
Annex A (informative) HPLC chromatogram of chloramphenicol standard solution and LC-MS/MS chromatogram of chloramphenicol standard solution
Bibliography