ISO 29981:2010
Milk products — Enumeration of presumptive bifidobacteria — Colony count technique at 37 degrees C
ISO 29981|IDF 220:2010 specifies a method for the selective enumeration of presumptive bifidobacteria in milk products by using a colony count technique at 37 °C under anaerobic conditions.
The method is applicable to milk products such as fermented and non-fermented milks, milk powders, infant formulae, and starter cultures where these microorganisms are present and viable, and in combination with other lactic acid bacteria.
Bifidobacteria used in milk products usually belong to the species: Bifidobacterium adolescentis; B. animalis subsp. animalis; B. animalis subsp. lactis; B. bifidum; B. breve; B. infantis; B. longum.
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Scope
Normative references
Terms and definitions
Principle
Culture media, diluents and reagents
Basic materials
Diluent(s)
Culture medium (TOS-MUP medium)
Basic medium (TOS-propionate agar medium, see Reference [10]
Composition
Transgalactosylated oligosaccharide mixture
Preparation
MUP supplement solution (see Reference [11])
Complete medium
Apparatus
Other suitable and safety-proven low-temperature catalyst sy
Sampling
Procedure
General
Preparation of the test portion and primary dilution
Dried milk products, e.g. infant milk formulae
Probiotic yogurt or yogurt-like products
Microscopic examination
Preparation of decimal dilutions
Inoculation
Duration of the procedure
Incubation
Counting of the colonies
Reading of the Petri dishes — confirmation
Calculation and expression of results
Calculation
Expression of results
Precision
Interlaboratory test
Repeatability
Reproducibility
Precision data collectively defined for dairy products
Knowledge of use of the method
Test report
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Related Information
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Scope
Normative references
Terms and definitions
Principle
Culture media, diluents and reagents
Basic materials
Diluent(s)
Culture medium (TOS-MUP medium)
Basic medium (TOS-propionate agar medium, see Reference [10]
Composition
Transgalactosylated oligosaccharide mixture
Preparation
MUP supplement solution (see Reference [11])
Complete medium
Apparatus
Other suitable and safety-proven low-temperature catalyst sy
Sampling
Procedure
General
Preparation of the test portion and primary dilution
Dried milk products, e.g. infant milk formulae
Probiotic yogurt or yogurt-like products
Microscopic examination
Preparation of decimal dilutions
Inoculation
Duration of the procedure
Incubation
Counting of the colonies
Reading of the Petri dishes — confirmation
Calculation and expression of results
Calculation
Expression of results
Precision
Interlaboratory test
Repeatability
Reproducibility
Precision data collectively defined for dairy products
Knowledge of use of the method
Test report