AS/NZS 4276.5:2007
Water microbiology - Method 5: Coliforms - Membrane filtration method
Extends the applicability of the membrane filtration method when used with samples containing different levels of competing non-target bacterial flora.
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AS/NZS 4276.5:2007 WATER MICROBIOLOGY - METHOD 5: COLIFORMS-MEMBRANE FILTRATION METHOD
PREFACE
METHOD
1 SCOPE
2 REFERENCED DOCUMENTS
3 DEFINITION
3.1 Coliforms
4 PRINCIPLE
5 CULTURE MEDIA AND REAGENTS (See Appendix B)
6 APPARATUS
6.1 Apparatus and materials for membrane filtration
6.2 Incubators or water baths
7 REFERENCE CULTURES
7.1 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or N捡牯渀.刀.......漀爀.愀.挀甀氀琀甀爀攀.琀爀愀挀攀愀戀氀攀.琀漀.愀渀礀.漀昀.琀栀攀猀攀.猀琀爀愀椀渀
7.2 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
7.3 Use of reference cultures
8 SAMPLES
8.1 Laboratory samples
8.2 Dilutions
9 PROCEDURE
9.1 Filtration procedure
9.2 Resuscitation and incubation
9.3 Identification of presumptive coliforms
9.4 Confirmatory tests
9.5 Interpretation of results
10 CALCULATION
11 TEST REPORT
12 BIBLIOGRAPHY
APPENDIX A - FLOW DIAGRAM FOR COLIFORMS—MEMBRANE FILTRATION
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 MEMBRANE LAURYL SULFATE (m-LS) AGAR OR BROTH
B3 m-ENDO AGAR OR BROTH
B4 NUTRIENT AGAR
B5 ORTHO-NITROPHENYL-β -D-GALACTOPYRANOSIDE (ONPG) BROTH
B6 OXIDASE REAGENT (KOVACS’)
APPENDIX C - PEFORMANCE OF KOVACS’ OXIDASE TEST
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Related Information
Australia New Zealand Food Standards Code Standard 1.6.1
Ministry: Food Standards Australia New Zealand
Section: 4.1(b)
Citation context:...for packaged water, packaged ice or mineral water - the relevant method prescribed by Australian New Zealand Standard AS/NZS 4276.
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AS/NZS 4276.5:2007 WATER MICROBIOLOGY - METHOD 5: COLIFORMS-MEMBRANE FILTRATION METHOD
PREFACE
METHOD
1 SCOPE
2 REFERENCED DOCUMENTS
3 DEFINITION
3.1 Coliforms
4 PRINCIPLE
5 CULTURE MEDIA AND REAGENTS (See Appendix B)
6 APPARATUS
6.1 Apparatus and materials for membrane filtration
6.2 Incubators or water baths
7 REFERENCE CULTURES
7.1 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or N捡牯渀.刀.......漀爀.愀.挀甀氀琀甀爀攀.琀爀愀挀攀愀戀氀攀.琀漀.愀渀礀.漀昀.琀栀攀猀攀.猀琀爀愀椀渀
7.2 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
7.3 Use of reference cultures
8 SAMPLES
8.1 Laboratory samples
8.2 Dilutions
9 PROCEDURE
9.1 Filtration procedure
9.2 Resuscitation and incubation
9.3 Identification of presumptive coliforms
9.4 Confirmatory tests
9.5 Interpretation of results
10 CALCULATION
11 TEST REPORT
12 BIBLIOGRAPHY
APPENDIX A - FLOW DIAGRAM FOR COLIFORMS—MEMBRANE FILTRATION
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 MEMBRANE LAURYL SULFATE (m-LS) AGAR OR BROTH
B3 m-ENDO AGAR OR BROTH
B4 NUTRIENT AGAR
B5 ORTHO-NITROPHENYL-β -D-GALACTOPYRANOSIDE (ONPG) BROTH
B6 OXIDASE REAGENT (KOVACS’)
APPENDIX C - PEFORMANCE OF KOVACS’ OXIDASE TEST
AS/NZS 4276.5:2007
Online Library subscription information
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