AS/NZS 3896:2008
Waters - Examination for Legionella spp. including Legionella pneumophila
Sets out a method for isolating and estimating the number of Legionella pneumophila and a range of other Legionella spp. in water.
| Get this standard | Prices exclude GST | |
|---|---|---|
| PDF ( Single user document) |
$94.50 NZD
|
|
| HardCopy |
$105.00 NZD
|
|
| Networkable PDF |
Price varies
|
|
| Online Library ( Annual subscription) |
Info
Price varies
Online Library subscription information Online library subscriptions provide a cost-effective solution for organisations of any size to access the latest standards, offering 24/7 access to a personalised catalogue. The service ensures subscribers have have the most up-to-date version of selected standards, incluing automatic updates for any amendments or versions. Pricing is dependent on the chosen standards and the number of concurrent users, with discounts applied from the standards PDF price. For customised subscriptions or further inquiries, contact the Online Library team. |
AS/NZS 3896:2008 WATERS-EXAMINATION FOR LEGIONELLA SPP. INCLUDING LEGIONELLA PNEUMOPHILA
PREFACE
CONTENTS
1 SCOPE
2 REFERENCED DOCUMENTS
3 PRINCIPLE
4 SAFETY PRECAUTIONS
5 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
5.1 Culture media
5.2 Reagents
5.2.1 Acid buffer
5.2.2 Gram stain reagents
5.2.3 Speciation system
5.3 Reference cultures
5.3.1 Positive cultures
5.3.2 Use of reference cultures
6 APPARATUS
6.1 Water bath at 50 ±1°C
6.2 Long-wave (360 nm approximately) ultraviolet lamp
6.3 Plate (or dissecting) microscope or hand lens, 10–20× magnification
6.4 Micropipette capable of delivering the required volumes, i.e. 0.1 mL and 0.01 mL
7 SAMPLE STORAGE
8 PROCEDURE
8.1 General
8.2 Isolation of Legionella-like organisms (LLOs)
8.2.1 Isolation procedure
8.2.2 Direct sample (untreated)
8.2.3 Heat-treated sample
8.2.4 Acid-treated sample
8.3 Incubation
8.4 Examination
8.5 Confirmation of Legionella spp
8.5.1 Procedure
8.5.2 Interpretation
8.5.3 Confirmation of Legionella pneumophila
8.5.4 Confirmation of Legionella spp other than L. pneumophila
9 CALCULATIONS
10 TEST REPORT
11 BIBLIOGRAPHY
APPENDIX A - FLOW CHART FOR LEGIONELLA SPP. TESTING
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 BLOOD AGAR (BA)
B3 BUFFERED CHARCOAL YEAST EXTRACT AGAR (BCYEα)
B3.1 Formulation
B3.2 Growth supplement
B4 BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITHOUT L-CYSTEINE (BCYE-Cys)
B5 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH BMPA SELECTIVE SUPPLEMENT (BMPAa)
B5.1 Formulation
B5.2 BMPA selective supplement
B6 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH MWY SELECTIVE SUPPLEMENT (MWY)
B6.1 Formulation
B6.2 MWY selective supplement
B7 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH GVPC SELECTIVE SUPPLEMENT (GVPC)
B7.1 Formulation
B7.2 GVPC selective supplement
B8 PREPARATION OF ACID BUFFER
B9 PEPTONE SOLUTION, 0.1%*
B10 RINGER’S SOLUTION, QUARTER STRENGTH
APPENDIX C - WORKED EXAMPLES
Previous versions
Keep me up-to-date
Sign up to receive updates when there are changes to this standard
Related Information
Building Code Compliance Schedule Handbook
Ministry: Ministry of Business, Innovation, and Employment - Building Systems Performance (BSP)
Section: SS9
Citation context:Similar Standards
-
AS/NZS 2031:2001
Selection of containers and preservation of water samples for microbiological analysis
-
AS/NZS 3666.1:2011
Air-handling and water systems of buildings - Microbial control - Part 1: Design, installation and commissioning -
AS/NZS 3666.2:2011
Air-handling and water systems of buildings - Microbial control - Part 2: Operation and maintenance
-
AS/NZS 3666.3:2011
Air-handling and water systems of buildings - Microbial control - Part 3: Performance-based maintenance of cooling water systems
AS/NZS 3896:2008 WATERS-EXAMINATION FOR LEGIONELLA SPP. INCLUDING LEGIONELLA PNEUMOPHILA
PREFACE
CONTENTS
1 SCOPE
2 REFERENCED DOCUMENTS
3 PRINCIPLE
4 SAFETY PRECAUTIONS
5 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
5.1 Culture media
5.2 Reagents
5.2.1 Acid buffer
5.2.2 Gram stain reagents
5.2.3 Speciation system
5.3 Reference cultures
5.3.1 Positive cultures
5.3.2 Use of reference cultures
6 APPARATUS
6.1 Water bath at 50 ±1°C
6.2 Long-wave (360 nm approximately) ultraviolet lamp
6.3 Plate (or dissecting) microscope or hand lens, 10–20× magnification
6.4 Micropipette capable of delivering the required volumes, i.e. 0.1 mL and 0.01 mL
7 SAMPLE STORAGE
8 PROCEDURE
8.1 General
8.2 Isolation of Legionella-like organisms (LLOs)
8.2.1 Isolation procedure
8.2.2 Direct sample (untreated)
8.2.3 Heat-treated sample
8.2.4 Acid-treated sample
8.3 Incubation
8.4 Examination
8.5 Confirmation of Legionella spp
8.5.1 Procedure
8.5.2 Interpretation
8.5.3 Confirmation of Legionella pneumophila
8.5.4 Confirmation of Legionella spp other than L. pneumophila
9 CALCULATIONS
10 TEST REPORT
11 BIBLIOGRAPHY
APPENDIX A - FLOW CHART FOR LEGIONELLA SPP. TESTING
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 BLOOD AGAR (BA)
B3 BUFFERED CHARCOAL YEAST EXTRACT AGAR (BCYEα)
B3.1 Formulation
B3.2 Growth supplement
B4 BUFFERED CHARCOAL YEAST EXTRACT MEDIUM WITHOUT L-CYSTEINE (BCYE-Cys)
B5 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH BMPA SELECTIVE SUPPLEMENT (BMPAa)
B5.1 Formulation
B5.2 BMPA selective supplement
B6 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH MWY SELECTIVE SUPPLEMENT (MWY)
B6.1 Formulation
B6.2 MWY selective supplement
B7 BUFFERED CHARCOAL YEAST EXTRACT AGAR WITH GVPC SELECTIVE SUPPLEMENT (GVPC)
B7.1 Formulation
B7.2 GVPC selective supplement
B8 PREPARATION OF ACID BUFFER
B9 PEPTONE SOLUTION, 0.1%*
B10 RINGER’S SOLUTION, QUARTER STRENGTH
APPENDIX C - WORKED EXAMPLES
Previous versions
AS/NZS 3896:2008
Online Library subscription information
Online library subscriptions provide a cost-effective solution for organisations of any size to access the latest standards, offering 24/7 access to a personalised catalogue. The service ensures subscribers have have the most up-to-date version of selected standards, incluing automatic updates for any amendments or versions. Pricing is dependent on the chosen standards and the number of concurrent users, with discounts applied from the standards PDF price. For customised subscriptions or further inquiries, contact the Online Library team.